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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Cysteine dioxygenase and taurine are essential for embryo implantation by involving in E2-ERα and P4-PR signaling in mouse

Fig. 4

Effects of Cdo deletion on PR and ER signaling transduction and the cell proliferation in the uteri of d 4 pregnancy mice. A, RT-qPCR analysis of relative Pgr mRNA expression levels. B and C, WB detection and analysis of PR protein expression. D, RT-qPCR analysis of relative Esr1 mRNA levels. E and F, WB detection and analysis of ERα protein expression levels. G, IHC staining of PR and ERα. Scale bars: 50 μm. H, Relative mRNA expressions of Ihh, Areg and Hand2. I, Relative mRNA levels of Ltf and Muc1. J, IF staining of Hand2 and Muc1. Scale bars: 50 μm. K, Ki67 (proliferative cell marker) IHC staining. Scale bars: 50 μm. GE: glandular epithelium. LE: luminal epithelium. St: stroma. Data are presented as Mean ± SEM, n ≥ 4. Different letters represent significant differences (P < 0.05)

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