Fig. 3

CDO deletion causes impairment of embryo implantation and severe subfertility. A and B, Serum (A) and uterus (B) taurine concentrations of pregnancy Cdo KO and WT females from d 1 to d 8, assayed by HPLC (n = 3). C, Pregnancy rates of WT and KO females. The number within brackets indicate females with pups over total number of plug-positive females. D, Average litter sizes of WT and Cdo KO females (n = 7). E, Representative images of d 5 pregnant uteri from WT and Cdo KO females. Bars, 1 cm. F, Blastocysts flushed from Cdo KO uterus. Bar, 50 μm. G, Average numbers of implanted embryos represented by implantation sites (n = 5). H, Representative patterns of d 5 WT receptive mice uteri transferred with WT and Cdo KO embryos. I, d 5 KO receptive mice uteri transferred with WT embryos. Arrowheads indicate the location of blastocysts. Bars, 1 cm. K, IS numbers of different treated mice uteri (n = 3). Data are presented as Mean ± SEM. Different letters above columns indicate significant differences (P < 0.05)