Fig. 2

Generation of Cdo KO mouse. A, Cas9n target scheme in the first and the third exons of Cdo gene. The blue lines indicate sgRNAs’ spacer and the red lines indicate PAM (protospacer adjacent motif). B, Sanger sequencing of the target region in F0 mice. The Cdo KO mice get a miss of 7489 nucleotides. C, Genotype identification results using PCR. The 325 bp strand indicates wild type and the 245 bp strand indicates mutant type. D, IHC staining of CDO in WT and Cdo KO mouse uterus. Bars, 50 μm. E, RT-qPCR analysis of Cdo mRNA levels in WT and Cdo KO mouse uteri. F, Representative patterns of WB analysis of CDO expressions. G-I, Taurine levels in WT and Cdo KO mice liver, serum and uterus detected by HPLC. Data are the Mean ± SEM. (n ≥ 3). Different letters above columns indicate significant differences (P < 0.05). GE: glandular epithelium. LE: luminal epithelium. Sm: smooth muscle cells. St: stromal cells. Scale bars: 50 μm