Table 2 Primer sequences for real-time PCR
Gene | GenBank ID | Primer sequence (5′ to 3′) |
|---|---|---|
ZO-1 | NM_040680628.1 | F: CCAAAGACAGCAGGAGGAGA |
R: TGGCTAGTTTCTCTCGTGCA | ||
Claudin-1 | NM_001013611.2 | F: GAGGATGACCAGGTCAAGAAG |
R: TGCCCAGCCAATGAAGAG | ||
Occludin | NM_205128.1 | F: TCATCCTGCTCTGCCTCATCT |
R: CATCCGCCACGTTCTTCAC | ||
MUC2 | NM_040673077.1 | F: CATTCAACGAGGAGAGCTGC |
R: TTCCTTGCAGCAGGAACAAC | ||
β-actin | NM_205518.1 | F: GAGAAATTGTGCGTGACATCA |
R: CCTGAACCTCTCATTGCCA |