Fig. 6

CTNNB1 promotes lipid accumulation of chicken preadipocytes. A and B The relative mRNA expression of CTNNB1 was detected by qPCR with CTNNB1 overexpressing and knockdown in chicken preadipocytes; C and D The relative protein expression of β-catenin was detected by western blot; E and F The relative protein expression of critical differentiation genes was detected with CTNNB1 overexpression and knockdown in chicken preadipocytes using western blot; G and H Oil red O staining results and quantification of lipid droplet in chicken preadipocytes after 48 h transfection of CTNNB1 overexpression plasmid and siRNA. These values are shown as mean ± SEM of at least three biological replicates. The statistical significance of the differences was assessed using the unpaired Student's t-test and one-way analysis of variance (ANOVA), followed by Tukey's multiple comparisons test. (*P < 0.05; **P < 0.01; ****P < 0.0001). Groups marked with the same letter were considered to have no significant difference, and those without the same letter were significantly different