Fig. 4
From: m6A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte
FTO promotes the adipogenesis of chicken preadipocytes. A and B The relative mRNA and protein levels of differentiation-related genes were detected in chicken preadipocytes transfected with overexpression plasmid of FTO using qPCR and western blot; C and D The relative mRNA and protein levels of differentiation-related genes were detected in chicken preadipocytes transfected with si-FTO and si-NC using qPCR and western blot; E Oil red O staining results and quantification of lipid droplet in chicken preadipocytes after 48 h transfection of FTO overexpression plasmid; F Oil red O staining results and quantification of lipid droplet in chicken preadipocytes after 48 h transfection of FTO siRNA. These values are shown as mean ± SEM of at least three biological replicates. The statistical significance of the differences was assessed using the unpaired Student's t-test (* P < 0.05; **P < 0.01; ****P < 0.0001)