Fig. 3

FTO promotes the proliferation of chicken preadipocytes. A and B CCK8 assay was used to detect cell proliferation rate with FTO overexpression and knockdown in chicken preadipocytes; C and D The relative mRNA expression of proliferation-related genes was detected after transfer with FTO overexpression plasmid and FTO siRNA in chicken preadipocytes using RT-PCR; E EdU staining assays for chicken preadipocyte transferred with pcDNA3.1-FTO and pcDNA3.1 for 48 h. F Fold change in proliferation rates of FTO overexpression using EdU assay. G EdU staining assays for chicken preadipocyte transferred with si-FTO and si-NC. H Fold change in proliferation rates of FTO knockdown using EdU assay. The values are shown as mean ± SEM of at least three biological replicates. The statistical significance of the differences was assessed using the unpaired Student's t-test (* P < 0.05; **P < 0.01)