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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: m6A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte

Fig. 2

Bioinformation analysis and demethylation effects of chicken FTO gene. A Phylogenetic tree of the FTO; B Analysis and annotation of FTO protein domains among different species; C and D The relative mRNA and protein expression of FTO were detected after transfer with pcDNA3.1-FTO and siRNA in chicken preadipocytes using RT-PCR and western blotting; E RNA m6A levels in chicken preadipocytes were detected after transfection of pcDNA3.1-FTO and siRNA by RNA dot blot and methylene blue staining. These values are shown as mean ± SEM of at least three biological replicates. The statistical significance of the differences was assessed using the unpaired Student's t-test (* P < 0.05; **P < 0.01; ***P < 0.001)

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