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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: m6A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte

Fig. 1

Betaine and cycloleucine supplementation affect global m6A levels and lipogenesis in chicken preadipocytes. A and B Detection of RNA m6A levels in chicken preadipocyte supplement with betaine and cycloleucine by RNA dot blot and methylene blue staining; C and D Cell proliferation rate by CCK8 treated with different doses of betaine and cycloleucine; E Cell proliferation analysis by flow cytometry EdU assay for betaine treatment; F The relative mRNA expression of proliferation-related genes of betaine treatment was detected in chicken preadipocyte; G Cell proliferation analysis by flow cytometry EdU assay for cycloleucine treatment; H The relative mRNA expression of proliferation-related genes of cycloleucine treatment was detected in chicken preadipocyte using qPCR; I The relative mRNA expression of adipogenic differentiation genes of betaine treatment was detected in chicken preadipocytes using qPCR; J The relative protein expression of PPARγ was detected by western blot in chicken preadipocytes treated with betaine; K The quantification of lipid droplet in chicken preadipocytes treated with different concentrations betaine; L The relative mRNA expression level of adipogenic differentiation genes of cycloleucine treatment was detected in chicken preadipocytes using qPCR; M The relative protein expression of PPARγ was detected by western blot in chicken preadipocytes treated with cycloleucine; N The quantification of lipid droplet in chicken preadipocytes treated with different concentrations betaine. The results of all groups are shown as mean ± SEM of at least three biological replicates. Statistical significances were assessed using one-way analysis of variance (ANOVA), followed by Tukey's multiple comparisons test. Groups marked with the same letter were considered to have no significant difference, and those without the same letter were significantly different

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