Table 1 Pig-specific primers used to detect colonic mucosa and liver-related genes
Gene name | Primers (5′ to 3′) | Amplicon size, bp | Accession No. |
|---|---|---|---|
ABST | F: AAGTTCCTGGGGCACGTAAA | 101 | NM_000452.3 |
R: CTCCTGGACAGCATCCCATT | |||
ATGL | F: ACCTGTCCAACCTGCTGC | 163 | NM_001098605.1 |
R: GCCTGTCTGCTCCTTTATCCA | |||
BESP | F: CGCAGCGTGAAGAAATGTGG | 126 | XM_003133457.5 |
R: AAAACCGAAACAGTTGAAAGAGGC | |||
CYP7A1 | F: GTGTTCAAGACGGGCCACTA | 120 | NM_001005352.3 |
R: GGAGCGACTTGGCTTTCTCT | |||
CYP8B1 | F: GCAGGCGGAGGAGTTATTCA | 130 | NM_214426.1 |
R: TTATGCCGTGCCTCTCCAAG | |||
CYP27A1 | F: TTGAGAAACGCATTGGCTGC | 90 | NM_001243304.1 |
R: ATCCAGGTATCGCCTCCAGT | |||
FAS | F: ATAAGTTCGACGTCAGCCCG | 88 | NM_001099930.1 |
R: TAGCCAGTGCCAGGGAAAAG | |||
FXR | F: TATGAACTCAGGCGAATGCCTGCT | 150 | NM_001287412.1 |
R: ATCCAGATGCTCTGTCTCCGCAAA | |||
FGF19 | F: TGAGTACCGTGGCGATCAAG | 108 | XM_003122420.3 |
R: GCGGATCTCCTCCTCGAAAG | |||
G6PC | F: AAGCCAAGCGAAGGTGTGAGC | 158 | NM_001113445.1 |
R: AAGCATTCAGCCAACA | |||
HSL | F: ACCCTCGGCTGTCAACTTCTT | 152 | NM_214315.3 |
R: TCCTCCTTGGTGCTAATCTCGT | |||
IBABP | F: GCAAGGAGTGCGACATAGAGAC | 102 | NM_001040442.1 |
R: TGGTGGTAGTTGGGGCTGTT | |||
MRP2 | F: GAACAGGTTTGCTGGCGATATT | 121 | XM_021073710.1 |
R: GCCAGGAGCGCAAAGACA | |||
NTCP | F: TGTCATCAAGGGAGGAACGA | 139 | XM_001927695.5 |
R: CGAGCATTGAGGCGGAAAAG | |||
PCK2 | F: ACAGGAGGTTCGTGACATTCGG | 172 | NM_001161753.1 |
R: GTGGTGCTGTGCTCACTTGCTA | |||
PC | F: CCGCAAGATGGGAGACAAGGT | 164 | NM_214349.1 |
R: GGAAGCCGTAGGTGTTGGAGAA | |||
TGR5 | F: TGATCATCGCTACACAGCCC | 124 | NM_001077191.2 |
R: TCAAGTCAAGGTCCACGCTG |