Dietary supplementation of Macleaya cordata extract and Bacillus in combination improve laying performance by regulating reproductive hormones, intestinal microbiota and barrier function of laying hens

Table 1 Ingredients and nutrient contents of the basal diet (as-fed basis)

Items	Value
Ingredients, %
Corn	57.00
Soybean meal (46% CP)	24.00
Wheat middling	5.50
Soybean oil	1.00
Limestone	9.00
Dicalcium phosphate	1.00
Salt	0.30
DL-methionine	0.12
Lysine-HCl	0.08
Premix^a	2.00
Total	100.00
Nutrient level^b
Metabolizable energy, Mcal/kg	2.70
Crude protein, %	16.43
Lysine, %	0.89
Methionine, %	0.40
Cysteine + methionine, %	0.75
Calcium, %	3.62
Total phosphorus, %	0.56
Available phosphorus, %	0.35

^aThe premix provided the following per kg of the diet: iron, 60 mg; copper, 10 mg; manganese, 80 mg; zinc 80 mg; iodine 0.3 mg; vitamin A, 12,500 IU; vitamin D₃, 4000 IU; vitamin K₃, 2 mg; thiamine, 1 mg; riboflavin, 8.5 mg; calcium pantothenate, 50 mg; niacin acid, 32.5 mg; pyridoxine, 8 mg; folic acid, 5 mg; B₁₂, 5 mg; choline chloride, 500 mg; phytase, 1000 IU
^bThe nutrient levels were calculated values

ISSN: 2049-1891