Pretreatment with probiotics Enterococcus faecium NCIMB 11181 attenuated Salmonella Typhimurium-induced gut injury through modulating intestinal microbiome and immune responses with barrier function in broiler chickens

Table 1 Composition and nutrient levels of the experimental basal diet (as-fed basis unless stated otherwise, %)

Items	Content	Items	Content
Ingredients, %		Calculated Nutrient levels^c
Corn (CP 8.0%)	61.00	Metabolizable energy, kcal/kg	2.9072
Soybean meal (CP 43.0%)	33.00	Crude protein, %	19.24
Soybean oil	2.00	Total calcium, %	0.97
Limestone-calcium carbonate	1.21	Available phosphorus, %	0.43
Calcium hydrogen phosphate	1.83	Lysine, %	1.03
Sodium chloride	0.30	Methionine, %	0.41
DL-Methionine (98%)	0.10
L-Lysine HCL (78%)	0.10
Vitamin premix^a	0.03
Mineral premix^b	0.20
Choline chloride (50%)	0.20
Ethoxyquin (33%)	0.03
Total, %	100.00

^aVitamin premix provided the following per kg of diets: vitamin A (retinylacetate), 12,500 IU; vitamin D₃(cholecalciferol), 2500 IU; vitamin E (DL-a-tocopherol acetate), 18.75 mg; vitamin K₃ (menadione sodium bisulfate), 2.65 mg; VB₁, 2 mg; VB₂, 6 mg; VB₆, 6 mg; vitamin B₁₂ (cyanocobalamin), 0.025 mg; biotin, 0.0325 mg; folic acid, 1.25 mg; pantothenic acid, 1.25 mg; nicotinic acid, 50 mg
^bMineral premix provided per kilogram of complete diet: Cu (as copper sulfate) 8 mg, Zn (as zinc sulfate) 75 mg, Fe (as ferrous sulfate) 80 mg, Mn (as manganese sulfate) 100 mg, Se (as sodium selenite) 0.15 mg, I (as potassium iodide) 0.35 mg
^cCalculated value based on the analyzed data of experimental diets

ISSN: 2049-1891