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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality

Fig. 2

OF and UF-EVs characterization. A Averaged finite track length adjustment Concentration/Size graphs for NTA of particles in fluid isolated from oviducts (left) and uterine horns (right). B Representative images from electron microscopy analyses of OF (left) and UF (right) samples. Some EVs and their sizes are indicated in the images. C Representative images of membranes treated for detection of EVs marker-proteins (CD9, HSP70 and ALIX) and EVs negative protein (CANX) in samples isolated from oviduct (OF-EVs) and uterus (UF-EVs). PC = positive controls (CD9 = blood, HSP70 = pancreas, ALIX = lung, CANX = urethra). MW = molecular weight marker. Numbers indicate the MW. Expected MW: CD9 = 22, 24 and 35 kDa, HSP70 = ~ 70 kDa, ALIX = 90 kDa and CANX = 90 kDa

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891