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Table 4 The qPCR validation of the RNA-seqa

From: Effects of lactic acid bacteria-fermented formula milk supplementation on ileal microbiota, transcriptomic profile, and mucosal immunity in weaned piglets

Genesb

RNA-seqc (LFM vs. CON)

Real-time PCRd (LFM vs. CON)

FC

P-value

FC

P-value

TNFRSF13C

0.46

0.037

0.36

0.048

CXCL10

0.50

0.024

0.42

0.026

FABP1

8.77

0.002

8.36

0.034

LTA

0.43

0.030

0.45

0.087

LYZ1

7.01

0.007

7.05

0.026

PIGR

6.02

0.013

6.14

0.042

SLC5A8

2.02

0.022

2.69

0.035

TFF2

5.06

0.001

5.02

0.004

  1. aData were presented as FC. The gene expressions were considered to be significantly altered when FC > 2 or < 0.5, P < 0.05
  2. bTNFRSF13C: tumor necrosis factor receptor superfamily member 13C; CXCL10: C-X-C motif chemokine 10; FABP1: fatty acid binding protein 1; LTA: lymphotoxin alpha; LYZ1: lysozyme-like protein 1; PIGR: polymeric immunoglobulin receptor; SLC5A8: sodium-coupled monocarboxylate transporter; TFF2: trefoil factor 2
  3. cResults based on RNA sequencing, n = 4
  4. dResults based on real-time PCR, n = 6
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891