Items | T = 0 h | T = 2 h | T = 4 h | P-value |
---|---|---|---|---|
Mean ± SD | Mean ± SD | Mean ± SD | ||
DNA decondensation (halo test) | ||||
 Sperm with increased DNA decondensation, % | 2.87 ± 1.56 | 3.03 ± 1.06 | 3.53 ± 1.78 | n.s. |
Sperm protamination (CMA3) | ||||
 Intensity of chromatin deprotamination, AU | 802.04 ± 41.03 | 853.12 ± 36.20* | 916.18 ± 35.83*# | < 0.0001 |
 Sperm with poor protamination, %CMA3+ | 16.76 ± 10.07 | 24.47 ± 7.68* | 29.71 ± 6.61*# | < 0.0001 |