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Table 2 Effects of post-thawing incubation time on sperm chromatin parameters

From: Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity

 Items

T = 0 h

T = 2 h

T = 4 h

P-value

Mean ± SD

Mean ± SD

Mean ± SD

DNA decondensation (halo test)

 Sperm with increased DNA decondensation, %

2.87 ± 1.56

3.03 ± 1.06

3.53 ± 1.78

n.s.

Sperm protamination (CMA3)

 Intensity of chromatin deprotamination, AU

802.04 ± 41.03

853.12 ± 36.20*

916.18 ± 35.83*#

< 0.0001

 Sperm with poor protamination, %CMA3+

16.76 ± 10.07

24.47 ± 7.68*

29.71 ± 6.61*#

< 0.0001

  1. n.s. non-statistically significant differences
  2. *Differences compared to 0 h (P < 0.05)
  3. #Differences compared to 2 h (P < 0.05)