Table 1 Effects of post-thawing incubation time on single- and double-stranded sperm DNA damage

  Neutral Comet OTM, AU

1.31 ± 0.20

1.44 ± 0.27

1.48 ± 0.29

n.s.

  Low DNA damage, %

99.96 ± 0.20

97.38 ± 1.95^*

97.11 ± 3.25^*

< 0.0001

  High DNA damage, %

0.04 ± 0.20

2.62 ± 1.95^*

2.89 ± 3.25^*

< 0.0001

  Alkaline Comet OTM, AU

15.57 ± 5.20

16.80 ± 5.59

28.38 ± 7.82^*#

< 0.0001

  Low DNA damage, %

74.12 ± 24.21

67.09 ± 27.04

24.97 ± 22.12^*#

< 0.0001

  Moderate DNA damage, %

25.05 ± 22.93

26.42 ± 21.20

55.41 ± 18.55^*#

< 0.001

  High DNA damage, %

0.83 ± 2.07

6.49 ± 10.46^*

19.62 ± 20.47^*#

< 0.0001

  Moderate + high DNA damage, %

25.88 ± 24.21

32.91 ± 27.04

75.03 ± 22.12^*#

< 0.0001