Stimbiotic supplementation modulated intestinal inflammatory response and improved broilers performance in an experimentally-induced necrotic enteritis infection model

Table 1 Composition of the basal diets

Item, %	Pre-starter, 1–7 d	Starter, 7–14 d	Grower, 14–21 d	Finisher, 21–30 d
Corn grain	41.09	45.72	49.46	49.61
Wheat	12.00	12.00	12.00	12.00
Rice pollards	2.40	2.50	2.50	2.50
Soybean meal (45%)	26.05	21.58	18.06	13.27
Sesame meal (47%)	1.00	2.00	2.00	3.00
DDGS (28%)	6.00	6.00	5.00	8.81
Animal proteins (50%)	6.57	5.11	5.60	5.48
Animal fats	1.70	2.00	2.10	2.30
L-Lysine (55%)	0.65	0.67	0.65	0.66
DL-Methionine (88%)	0.38	0.34	0.35	0.35
L-Threonine (98%)	0.16	0.15	0.14	0.13
L-Tryptophan (98%)	0.01	0.03	0.10	0.10
Salt-proc (Fine)	0.21	0.20	0.20	0.21
Limestone	1.28	1.22	1.41	1.13
Mineral premix¹	0.20	0.20	0.20	0.20
Vitamin premix²	0.13	0.11	0.10	0.10
Choline, 50%	0.10	0.10	0.08	0.08
Phytase1000	0.09	0.07	0.06	0.07
Calculated value, %
AME, kcal/kg	3018	3030	3080	3110
Moisture	11.804	11.949	11.878	11.777
C Protein	23.402	21.155	20.112	19.508
Lysine	1.481	1.323	1.234	1.149
Methionine	0.739	0.664	0.667	0.674
TSAA	1.116	1.019	1.021	1.015
Threonine	0.996	0.909	0.857	0.809
Tryptophan	0.252	0.223	0.214	0.203
C Fat	5.539	5.824	6.079	6.515
C Fiber	3.151	3.121	2.943	3.145
C Ash	5.727	5.325	5.087	4.893
Calcium	0.850	0.800	0.750	0.700
Phosphorus	0.630	0.631	0.601	0.536

¹Provided per kg of diet: 37.5 mg Zn (as ZnSO₄), 37.5 mg of Mn (MnO₂), 37.5 mg of Fe (as FeSO₄•7H₂O), 3.75 mg of Cu (as CuSO₄•5H2O), 0.83 mg of I (as KI), and 0.23 mg of Se (as Na₂SeO₃•5H₂O)
²Provided per kg of diet: 15,000 IU of vitamin A, 3,750 IU of vitamin D₃, 37.5 mg of vitamin E, 2.55 mg of vitamin K₃, 3 mg of thiamin, 7.5 mg of riboflavin, 4.5 mg of vitamin B₆, 24 μg of vitamin B₁₂, 51 mg of niacin, 1.5 mg of folic acid, 0.2 mg of biotin and 13.5 mg of pantothenic acid

ISSN: 2049-1891