Table 2 Primers sequences of qPCR
Gene name | Direction | Primer sequences (5’ to 3’) | Concentration, μmol/L | Reaction efficiency, % |
|---|---|---|---|---|
IL-1β | F | CCTCGGTTCCATGGGAGATG | 10 | 93.6 |
| Â | R | AGGCACTGTTCCTCAGCTTC | 10 | Â |
IL-18 | F | TCAGATAATGCACCCCAGACC | 10 | 91.3 |
| Â | R | GATGGTTACGGCCAGACCTC | 10 | Â |
NLRP3 | F | ATGGTAAGTGTCCGCTGCAA | 10 | 95.5 |
| Â | R | ATGCCTTCTCTTCCCCGTTG | 10 | Â |
ZO-1 | F | CACTATGACCCCGAGGAGGA | 10 | 98.1 |
| Â | R | AACTGTGGCTGAGATTGGGG | 10 | Â |
Occludin | F | CCATCGGAGTTTCAGGTGAATG | 10 | 91.2 |
| Â | R | CTCCGCCTGAAGAAGCAGAAA | 10 | Â |
GAPDH | F | GTCTTCACTACCATGGAGAAGG | 10 | 94.4 |
| Â | R | TCATGGATGACCTTGGCCAG | 10 | Â |