Fig. 7

NLRP3 knockdown attenuated inflammatory responses. MAC-T cells were transfected with NLRP3 siRNA for 5 h and/or treated with BC (MOI = 5) and/or LGR-1 (MOI = 100) for another 3 h. (A) NLRP3 mRNA level. (B) Protein level of NLRP3. (C) Relative protein level of NLRP3. (D) Protein level of Caspase-1 p20. (E) IL-1β mRNA level. (F) IL-18 mRNA level. (G) Cell death in MAC-T. (H) Relative protein level of Caspase1 p20. In A and C, data for the si-CONTROL group were used to normalize data of each treatment group. Comparisons among groups were calculated using the t-test. Data are means ± SEM of three independent experiments. In E–H, the data for the CONTROL group were used to normalize the data of each treatment group. Comparisons among groups were calculated using one-way ANOVA. Data are means ± SEM of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001