Fig. 6

BC induced NLRP3 inflammasome activity. MAC-T cells were treated with BC (MOI = 5) and/or MCC950 (100 nmol/L). (A) Protein levels of NLRP3, Caspase-1 p20, GSDMD p30. (B) IL-1β mRNA level. (C) IL-18 mRNA level. (D) Relative protein level of NLRP3. (E) Relative protein level of Caspase-1 p20. (F) Relative protein level of GSDMD p30. The data for the CONTROL group were used to normalize the data of each treatment group. Comparisons among groups were calculated using one-way ANOVA. Data are means ± SEM of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001