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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Lactobacillus rhamnosus GR-1 attenuates foodborne Bacillus cereus-induced NLRP3 inflammasome activity in bovine mammary epithelial cells by protecting intercellular tight junctions

Fig. 3

LGR-1 attenuated BC-induced NLRP3 inflammasome activity. MAC-T cells were treated with BC (MOI = 5) and/or LGR-1 (MOI = 100) for 3 h. (A) and (B) Protein levels of NLRP3, ASC, Caspase-1 p20, GSDMD p30. (C) IL-1β and IL-18 mRNA level. Expression of NLRP3, ASC, GSDMD, and Caspase-1 in cells measured by immunofluorescence analysis; scale bar shown in the lower right corner. (D) NLRP3. (E) ASC. (F) GSDMD. (G) Caspase-1. The data for the CONTROL group were used to normalize the data of each treatment group. Comparisons among groups were calculated using one-way ANOVA. Data are means ± SEM of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891