Fig. 2

LGR-1 protects BC damaged intercellular tight junctions. MAC-T cells were treated with BC (MOI = 5) or LPS (500 ng/mL) + ATP (5 mmol/L) and/or LGR-1 (MOI = 100). (A) The ultrastructure of LGR-1 and/or BC treated cells was visualized by TEM. The scale bar is shown in the lower right corner. (B) Expression of ZO-1 in cells measured by immunofluorescence analysis; the scale bar is shown in the lower right corner. (C) and (F) Protein levels of ZO-1 and occludin in MAC-T. (D) and (G) Relative protein level of ZO-1. (E) and (H) Relative protein level of occludin. The data for the CONTROL group were used to normalize the data of each treatment group. White arrows indicate the nuclear membrane and black arrowheads indicate junctional complexes. Comparisons among groups were calculated using one-way ANOVA. Data are means ± SEM of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001