Table 5 The free amino acid composition (mg/100 g tissue) and IMP content (nmol/L) in the muscle of grass carp

Aspartate

0.20 ± 0.17^a

0.74 ± 0.72^ab

0.97 ± 0.43^b

0.92 ± 0.30^b

0.58 ± 0.28^ab

0.51 ± 0.46^ab

0.50

0.49

10.84

 < 0.01

Glutamate

6.41 ± 1.93^a

9.69 ± 2.93^b

6.79 ± 1.96^ab

7.53 ± 1.69^ab

7.39 ± 3.90^ab

8.38 ± 1.15^ab

0.20

0.66

0.00

0.97

Glutamine

56.51 ± 14.78^a

63.74 ± 10.21^ab

73.04 ± 4.20^b

65.89 ± 6.25^ab

63.39 ± 17.31^ab

65.94 ± 12.29^ab

0.94

0.34

2.58

0.12

Glycine

189.08 ± 31.76^b

196.48 ± 44.21^b

131.06 ± 26.76^a

103.88 ± 17.22^a

101.95 ± 22.60^a

97.33 ± 12.35^a

65.65

 < 0.01

3.47

0.07

Alanine

73.95 ± 7.62^d

56.37 ± 7.05^c

40.97 ± 9.41^b

42.00 ± 8.77^b

37.98 ± 7.82^ab

29.00 ± 8.03^a

100.33

 < 0.01

8.42

 < 0.01

Proline

253.51 ± 54.40^a

296.52 ± 64.87^ab

313.76 ± 48.70^ab

336.06 ± 57.28^b

288.66 ± 43.10^ab

270.17 ± 45.34^ab

0.21

0.65

8.21

 < 0.01

Threonine

41.69 ± 8.24^a

52.61 ± 9.06^ab

60.30 ± 12.22^b

56.40 ± 10.53^b

51.20 ± 4.70^ab

52.12 ± 10.69^ab

1.82

0.19

8.09

 < 0.01

Methionine

1.13 ± 0.44^a

1.78 ± 0.66^abc

2.38 ± 1.38^bc

2.88 ± 0.57^c

2.21 ± 0.82^abc

1.68 ± 1.23^ab

2.08

0.16

10.32

 < 0.01

Valine

5.49 ± 1.54^ab

5.59 ± 1.53^ab

5.31 ± 0.71^a

7.31 ± 1.22^bc

8.61 ± 1.13^c

8.58 ± 2.42^c

26.06

 < 0.01

1.00

0.32

Isoleucine

2.64 ± 0.45^a

2.78 ± 0.75^a

2.77 ± 0.39^a

3.05 ± 0.65^a

4.99 ± 1.24^b

4.82 ± 1.41^b

33.60

 < 0.01

3.42

0.07

Phenylalanine

3.19 ± 0.79^b

2.58 ± 0.71^ab

2.32 ± 0.71^a

4.28 ± 0.86^b

4.41 ± 0.86^b

4.96 ± 0.77^b

36.88

 < 0.01

6.27

 < 0.05

Leucine

15.24 ± 2.48^ab

13.24 ± 2.95^a

12.96 ± 4.10^a

16.74 ± 2.73^ab

19.67 ± 3.91^b

19.58 ± 4.51^b

13.80

 < 0.01

2.88

0.10

Tyrosine

4.48 ± 0.71^c

2.11 ± 0.61^a

2.21 ± 0.51^a

2.70 ± 0.68^ab

3.20 ± 1.04^b

3.39 ± 0.98^b

0.40

0.53

24.44

 < 0.01

Arginine

57.46 ± 13.40^ab

93.46 ± 33.77^b

95.85 ± 56.11^b

76.72 ± 32.40^ab

57.47 ± 8.92^ab

43.43 ± 13.02^a

3.47

0.07

8.43

 < 0.01

Serine

16.56 ± 3.54

14.19 ± 2.91

14.86 ± 5.71

13.32 ± 3.42

13.19 ± 4.42

12.53 ± 3.03

3.33

0.08

0.13

0.72

Lysine

50.46 ± 8.98^a

55.37 ± 15.72^a

69.94 ± 17.71^a

104.92 ± 26.61^b

116.01 ± 27.05^b

154.55 ± 24.59^c

104.12

 < 0.01

3.80

0.06

Histidine

446.84 ± 67.81^a

547.10 ± 73.00^b

557.44 ± 49.11^b

621.42 ± 82.94^bc

661.77 ± 51.30^c

566.86 ± 80.47^b

18.45

 < 0.01

11.08

 < 0.01

TAA

1225.27 ± 75.89^a

1414.85 ± 149.59^b

1393.32 ± 146.26^b

1466.31 ± 107.43^b

1443.14 ± 55.46^b

1344.20 ± 107.23^ab

3.85

0.06

11.90

 < 0.01

IMP

48.03 ± 4.82^a

56.24 ± 3.74^bc

57.67 ± 5.05^bc

61.01 ± 5.48^c

51.88 ± 5.94^ab

51.22 ± 6.05^ab

0.12

0.73

19.53

 < 0.01