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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: Bacillus licheniformis PF9 improves barrier function and alleviates inflammatory responses against enterotoxigenic Escherichia coli F4 infection in the porcine intestinal epithelial cells

Fig. 5

Influence of B. licheniformis PF9 on ETEC F4-induced gene and protein expressions of tight junctions (TJs) in IPEC-J2 cells. A Total RNA was extracted from IPEC-J2 cells and the gene expressions of ZO-1, OCLN, CLDN1, and CLDN3 were detected by qPCR (n = 4). B Total protein was extracted from IPEC-J2 cells and the protein expressions of ZO-1, OCLN, and CLDN3 were detected by western blotting (n = 3). The data were presented as mean ± SD. Different letters represent a significant difference (P < 0.05)

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