Table 6 The effect on replacing alfalfa silage with paulownia leaves silage (60 g/kg) on fatty acid (FA) proportion (g/100 g of FA) in ruminal fluid (n = 4) (Exp. 2)

C8:0

0.13^a

0.092^b

0.11^a

0.089^b

0.088^b

0.11^a

0.11

0.10

0.006

0.02

0.21

 < 0.01

C10:0

0.13

0.10

0.14

0.10

0.13

0.10

0.13

0.10

0.005

 < 0.01

0.72

0.98

C12:0

0.44^a

0.10^b

0.45^a

0.10^b

0.38^a

0.10^b

0.42

0.10

0.019

 < 0.01

0.02

0.04

C14:0

1.12

1.05

1.10

0.96

0.93

0.95

1.05

0.98

0.03

0.04

 < 0.01

0.12

C14:1

1.18^b

1.92^a

1.14^b

1.89^a

1.16^b

1.58^a

1.16

1.80

0.05

 < 0.01

 < 0.01

 < 0.01

C15:0

1.17^b

1.52^a

1.10^b

1.54^a

1.12^b

1.44^a

1.13

1.50

0.02

 < 0.01

0.01

0.01

C15:1

0.46^b

0.77^a

0.47^b

0.73^a

0.49^b

0.66^a

0.47

0.73

0.02

 < 0.01

0.01

 < 0.01

C16:0

20.4^a

19.0^b

19.7^b

19.5^b

20.6^a

19.3^b

20.3

19.2

0.15

 < 0.01

0.23

0.02

C16:1

0.48^a

0.22^b

0.46^a

0.22^b

0.32^a

0.23^b

0.42

0.22

0.002

 < 0.01

0.01

 < 0.01

C17:0

0.56

0.60

0.52

0.62

0.52

0.59

0.54

0.60

0.009

 < 0.01

0.08

0.07

C17:1

0.12

0.079

0.10

0.10

0.12

0.087

0.11

0.087

0.005

 < 0.01

0.95

0.10

C18:0

47.8

45.9

47.4

46.7

46.9

45.1

47.4

45.9

0.42

 < 0.01

0.17

0.55

C18:1 trans-10

1.36^b

2.51^a

1.23^b

2.57^a

1.31^b

2.78^a

1.30

2.62

0.07

 < 0.01

 < 0.01

 < 0.01

C18:1 trans-11

0.41

0.67

0.46

0.70

0.46

0.74

0.45

0.70

0.02

 < 0.01

 < 0.01

0.36

C18:1 cis-9

5.67

5.78

6.06

5.56

6.64

5.96

6.13

5.76

0.14

0.03

 < 0.01

0.21

C18:2 cis-9 cis-12

5.27^c

7.38^a

6.52^b

6.78^b

6.74^b

7.47^a

6.14

7.19

0.14

 < 0.01

 < 0.01

 < 0.01

C18:2 cis-9 trans-11

0.16^b

0.86^a

0.24^b

0.87^a

0.21^b

0.87^a

0.20

0.87

0.03

 < 0.01

 < 0.01

0.03

C18:2 trans-10 cis-12

0.12^b

0.16^a

0.15^b

0.14

0.15^b

0.17^a

0.14

0.16

0.005

 < 0.01

 < 0.01

 < 0.01

C18:3 n-6

0.16

0.17

0.16

0.17

0.16

0.19

0.16

0.18

0.005

 < 0.01

0.21

0.61

C18:3 cis-9 cis-12 cis-15

1.29

1.38

1.15

1.34

1.23

1.34

1.23

1.35

0.02

 < 0.01

0.01

0.27

Sum of other FA⁴

9.07^c

9.83^a

9.80^a

9.42^b

9.48^b

9.90^a

9.44

9.71

0.12

0.08

0.36

 < 0.01

Sum of SFA

74.5^a

69.4^b

73.0^a

70.5^ab

71.9^a

68.7^b

73.2

69.5

0.36

 < 0.01

 < 0.01

0.02

Sum of UFA

25.5^c

30.6^a

27.0^b

29.5^ab

28.1^b

31.3^a

26.8

30.5

0.36

 < 0.01

 < 0.01

0.02

Sum of MUFA

17.3

20.9

17.3

20.4

17.9

20.9

17.5

20.7

0.28

 < 0.01

0.27

0.65

Sum of PUFA

8.30^b

9.73^a

9.47^a

8.98^b

9.56^a

10.1^a

9.08

9.59

0.15

 < 0.01

 < 0.01

 < 0.01

Sum of n-6 FA

6.47^c

8.43^a

8.13^ab

7.85^b

8.33^a

8.98^a

7.60

8.41

0.17

 < 0.01

 < 0.01

 < 0.01

Sum of n-3 FA

1.29

1.40

1.15

1.32

1.23

1.34

1.23

1.35

0.02

 < 0.01

 < 0.01

0.58

Sum of trans C18:1

2.38^b

4.99^a

2.33^b

5.03^a

2.37^b

5.34^a

2.36

5.11

0.14

 < 0.01

 < 0.01

 < 0.01

Sum of medium-chain FA

26.2

26.9

25.3

26.8

25.9

26.3

25.9

26.7

0.20

 < 0.01

0.23

0.09

Sum of long-chain FA

73.6

73.0

74.0

73.0

73.8

73.5

73.8

73.1

0.21

0.01

0.46

0.45

n-6/n-3 FA ratio

5.13^e

5.57^de

7.80^a

6.05^c

6.65^b

6.69^b

6.44

6.09

0.19

0.05

 < 0.01

 < 0.01

PUFA/SFA ratio

0.11^c

0.14^a

0.13^b

0.13^b

0.13^b

0.15^a

0.13

0.14

0.003

 < 0.01

 < 0.01

 < 0.01