Fig. 9

PCB2 and ConA exert different roles in mitochondrial function. A Mitochondrial membrane potential was detected by JC-1 staining. ConA, PCB2 and ML-7 were supplemented in recovery medium for 1 h during warming process. Scale bar = 50 μm. B Quantification of the MMP level in different groups. C ATP was measured in different groups. D Representative images of mitochondria distribution in different groups. Oocytes were stained with Mito-Tracker Green. Scale bar = 50 μm. E Rate of abnormal mitochondria distribution in different groups. F pERM staining of matured oocytes. DNA was counterstained with DAPI (blue). Scale bar = 50 μm. G Relative fluorescence intensity of pERM signals was recorded in different groups. “n” represents the cell number used in this experiment. Data are presented as mean percentage (mean ± SEM) of at least three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns non significance