Fig. 7

F-actin and spindle migration after vitrification. A The distance between the spindle pole to the cortex (Length, L) and the diameter (D) of oocyte was quantified to determine the cortically and centrally positioned spindles. Scale bar = 50 μm. B Rate of L/D in different groups. C Representative images of F-actin in different groups. Scale bar = 50 μm. D Quantification of F-actin fluorescence intensity. “n” represents the cell number used in this experiment. Data are presented as mean percentage (mean ± SEM) of at least three independent experiments. ^**P < 0.01, ^***P < 0.001