Fig. 10

PCB2 was mainly involved in the regulation of glycometabolism during embryo development. A Immunofluorescent staining of CDX2 and Nanog in blastocysts from different groups. DNA was counterstained with DAPI (blue). Scale bar = 50 μm. B-C The ICM: TE rate and ICM: total cells rate were compared in different groups. D Schematic diagram of metabolomic sample collection. E–H The relative contents of four amino acids (L-hydroxyproline, L-citrulline, L-kynurenine hydrate, and L-glutamic acid) in different groups. I-M The mean contents of five carbohydrates (arabinose, fructose, glucose, maltose, and xylose) in the medium of different groups. “n” represents the cell number used in this experiment. Data are presented as mean percentage (mean ± SEM) of at least three independent experiments. ^***P < 0.001, ns non significance