Effect of the dietary supplementation with extracts of chestnut wood and grape pomace on performance and jejunum response in female and male broiler chickens at different ages

Table 5 Jejunum morphometry, number of goblet cells and densities of CD45⁺ and CD3⁺ cells at 15 and 35 days of age

Items	Diet (D)			Age (A)		Sex (S)		P-value							MSE
Items	C	CN	GP	15	35	F	M	D	A	S	D×A	A×S¹	D×S²	D×A×S	MSE
Broilers, n	24	24	24	36	36	36	36
Villi height, μm	1033^b	934^a	954^ab	866	1082	936	1011	<0.001	<0.01	<0.01	0.44	0.02	0.03	0.13	111
Crypt depth, μm	145	139	144	138	147	140	145	0.45	0.04	0.23	0.70	0.29	0.79	0.31	18,5
Villi / Crypt ratio	7.49	7.06	6.96	6.67	7.67	7.06	7.28	<0.001	0.16	0.37	0.70	0.37	0.20	0.68	1,00
Goblet cells, n/300 μm	21.02	21.67	22.25	22.84	20.45	22.20	21.09	0.24	0.001	0.06	0.34	0.12	0.43	0.27	2.46
CD3⁺ cells, n/10,000 μm²	2242	2219	2297	2069	2436	2223	2283	0.16	<0.001	0.09	0.23	<0.001	<0.001	0.46	459
CD45⁺ cells, n/10,000 μm²	2879^ab	2793^a	2925^b	2437	3295	2915	2816	0.02	<0.001	0.01	0.18	<0.001	0.01	0.45	537

MSE root mean square error. C, control diet. CN, control diet supplemented with 0.2% chestnut wood extracts. GP, control diet supplemented with 0.2% grape pomace extracts
¹Averages of traits according to Age × Sex are provided in Table S1. ²Averages of traits according to Diet × Sex are provided in Table S2.
^a,bValues with different superscript letters significantly differ (P < 0.05)

ISSN: 2049-1891