Lipid metabolism and m6A RNA methylation are altered in lambs supplemented rumen-protected methionine and lysine in a low-protein diet

Table 5 Effect of a LP diet supplemented with rumen-protected methionine and lysine on the fatty acids composition of muscle in growing lambs, % of total fatty acids

Fatty acids, %	Treatment			SEM	P-value
Fatty acids, %	NP	LP	LP + RML	SEM	P-value
C12:0	0.627	0.769	0.482	0.132	0.174
C14:0	2.895	3.080	3.003	0.167	0.754
C14:1	0.227	0.217	0.235	0.014	0.890
C15:0	0.981^a	0.678^b	0.715^b	0.037	< 0.001
C16:0	23.95	24.72	25.01	0.641	0.503
C16:1	2.222	2.234	2.210	0.115	0.988
C17:0	1.182	0.976	0.923	0.087	0.288
C18:0	18.03	17.90	17.24	0.859	0.794
C18:1n-9 t	0.636^b	0.750^a	0.501^b	0.045	0.006
C18:1n-9c	39.06	39.11	39.65	0.616	0.759
C18:2n-6c	5.988	5.450	5.860	0.216	0.256
C20:0	0.318	0.293	0.259	0.034	0.470
C18:3n-3	2.369^a	2.206^b	2.209^b	0.041	0.018
C20:3n-6	0.260	0.247	0.286	0.024	0.515
C20:4n-6	0.301^b	0.448^a	0.306^b	0.023	< 0.001
C24:0	0.330	0.347	0.369	0.016	0.231
C22:6n-3	0.619	0.578	0.594	0.080	0.868
Fatty acid partial sums
SFA¹	48.31	48.76	48.00	0.751	0.769
MUFA²	42.15	42.31	42.59	0.644	0.883
PUFA³	9.538	8.930	9.400	0.259	0.353
∑n-6 PUFA⁴	6.550	6.145	6.595	0.244	0.466
∑n-3 PUFA⁵	2.988	2.785	2.804	0.062	0.066

Different letters across a row indicate a significant difference among the three dietary treatments (P < 0.05). Data are presented as means with their pooled SEM. NP, adequate protein diet (n = 7); LP, low-protein diet (n = 6); LP + RML, low-protein diet supplemented with rumen-protected methionine and lysine (n = 7)
¹SFA (saturated fatty acid) = C12:0 + C14:0 + C15:0 + C16:0 + C17:0 + C18:0 + C20:0 + C24:0
²MUFA (monounsaturated fatty acid) = C14:1 + C16:1 + C18:1n-9 t + C18:1n-9c
³PUFA (polyunsaturated fatty acid) = C18:2n-6c + C18:3n-3 + C20:3n-6 + C20:4n-6 + C22:6n-3
⁴n-3 PUFA = C18:3n-3 + C22:6n-3
⁵n-6 PUFA = C18:2n-6c + C20:3n-6 + C20:4n-6

ISSN: 2049-1891