Fig. 3

Glyphosate exposure affects ER functions in porcine oocytes. A Representative images of ER distribution in control and 400 μmol/L glyphosate exposure oocytes. Bar = 30 μm. B The relative florescence intensity of ER in control and 400 μmol/L glyphosate-exposed oocytes. **, P < 0.01. C Representative images of Ca²⁺ in control and 400 μmol/L glyphosate-exposed groups. Bar = 30 μm. D The relative florescence intensity of Ca²⁺ after 400 μmol/L glyphosate treatment. *, P < 0.05. E Relative expression of ER-related genes was measured in control and 400 μmol/L glyphosate-exposed oocytes. *, P < 0.05; **, P < 0.01. F The protein expression of GRP78 in the control and 400 μmol/L glyphosate-exposed oocytes. *, P < 0.05