Fig. 2

Glyphosate exposure impairs mitochondria functions in porcine oocytes. A Representative images of mitochondria in control and 400 μmol/L glyphosate-exposed oocytes. Bar = 30 μm. B The relative fluorescence intensity of mitochondria after 400 μmol/L glyphosate exposure. *, P < 0.05. C Relative mtDNA copy number was assessed in control and 400 μmol/L glyphosate-exposed oocytes. *, P < 0.05. D Expression of mitochondrial-related genes was detected by RT-PCR in the control and 400 μmol/L glyphosate-exposed oocytes. *, P < 0.05. E Representative images of ROS signals in control and 400 μmol/L glyphosate-exposed oocytes. Bar = 100 μm. F The relative fluorescence intensity of ROS was analyzed in control and 400 μmol/L glyphosate-exposed oocytes. **, P < 0.01. G Relative expression of antioxidant-related genes in control and 400 μmol/L glyphosate-exposed oocytes. *, P < 0.05