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Fig. 9 | Journal of Animal Science and Biotechnology

Fig. 9

From: Integrative analysis of miRNA and mRNA profiles reveals that gga-miR-106-5p inhibits adipogenesis by targeting the KLF15 gene in chickens

Fig. 9

Effects of KLF15 on the proliferation of chicken abdominal preadipocytes. A CCK8 assay of chicken abdominal preadipocytes transfected with pcDNA3.1-KLF15-EGFP and pcDNA3.1-EGFP at 12, 24, 48, 72, and 96 h post-transfection; (B) CCK8 assay of chicken abdominal preadipocytes transfected with siKLF15 and siNC at 12, 24, 48, 72, and 96 h post-transfection; (C) Representative images from the EdU assay of chicken abdominal preadipocytes transfected with pcDNA3.1-EGFP and pcDNA3.1-KLF15-EGFP at 48 h post-transfection; (D) Representative images from the EdU assay of chicken abdominal preadipocytes transfected with siNC and siKLF15 at 48 h post-transfection; (E) mRNA expression levels of KLF15, PCNA, MKI67, and CDK1 after KLF15 overexpression; (F) mRNA expression levels of KLF15, PCNA, MKI67, and CDK1 after KLF15 knockdown

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891