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Fig. 7 | Journal of Animal Science and Biotechnology

Fig. 7

From: Integrative analysis of miRNA and mRNA profiles reveals that gga-miR-106-5p inhibits adipogenesis by targeting the KLF15 gene in chickens

Fig. 7

Validation of the KLF15 gene as a direct target of gga-miR-106-5p. A Potential gga-miR-106-5p binding sites in the 3′ UTR of the KLF15 gene; (B) Construction and validation of dual-luciferase reporter vectors for the validation of gga-miR-106-5p targeting the KLF15 gene. The sequences with or without the binding sites of gga-miR-106-5p and the 3′ UTR of the KLF15 gene were cloned into the psiCHECK-2 vector. WT: wild-type vector; mut: mutant vector; hRluc: Renilla luciferase; hluc+: firefly luciferase; (C, D) Validation of the interaction between gga-miR-106-5p and the 3′ UTR of the KLF15 gene via a dual-luciferase reporter assay in 293 T cells and DF1 cells. Data are presented as mean ± SD (n = 3)

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