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Fig. 10 | Journal of Animal Science and Biotechnology

Fig. 10

From: Integrative analysis of miRNA and mRNA profiles reveals that gga-miR-106-5p inhibits adipogenesis by targeting the KLF15 gene in chickens

Fig. 10

Effects of KLF15 on the adipogenic differentiation of chicken abdominal preadipocytes. A Oil red O staining of the differentiated chicken abdominal adipocytes transfected with pcDNA3.1-EGFP and pcDNA3.1-KLF15-EGFP; (B) Representative images of Nile red fluorescent staining of the differentiated chicken abdominal adipocytes transfected with pcDNA3.1-EGFP and pcDNA3.1-KLF15-EGFP; (C) Spectrophotometric analysis of lipid droplet content via Oil red O staining of the differentiated chicken abdominal adipocytes transfected with pcDNA3.1-EGFP and pcDNA3.1-KLF15-EGFP; (D) mRNA expression levels of KLF15, PPARγ, CEBPα, SLC27A1, ACSL1, ACACA, FASN, and AGPAT2 in the differentiated chicken abdominal adipocytes transfected with pcDNA3.1-EGFP and pcDNA3.1-KLF15-EGFP; (E) Oil red O staining of the differentiated chicken abdominal adipocytes transfected with siNC and siKLF15; (F) Representative images of Nile red fluorescent staining of the differentiated chicken abdominal adipocytes transfected with siNC and siKLF15; (G) Spectrophotometric analysis of lipid droplet content via Oil red O staining of the differentiated chicken abdominal adipocytes transfected with siNC and siKLF15; (H) mRNA expression levels of KLF15, PPARγ, CEBPα, SLC27A1, ACSL1, ACACA, FASN, and AGPAT2 in the differentiated chicken abdominal adipocytes transfected with siNC and siKLF15

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