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Table 1 Ingredient composition (g/100 g) and proximate (% of fed) of the test diets used for European seabass subadults

From: A plant-based diet supplemented with Hermetia illucens alone or in combination with poultry by-product meal: one step closer to sustainable aquafeeds for European seabass

Ingredient composition

CV

VH10

VH10P30

FH10

CF

Fish meal1

16

16

Fish meal2

4

4

4

45

45

Vegetable-protein mix 13

41

33.6

13.1

6

Vegetable-protein mix 24

20

20

20

  

Hermentia illucens meal5

8

8

8

PBM6

20.2

Feeding stimulants7

5.5

5.5

5.5

Wheat meal*

1

2.6

6.7

8.2

5.7

Whole peas*

6.7

6

5

8

11

Fish oil8

6

5.6

6

7.5

8

Vegetable oil mix9

11.5

10.4

7.7

5.4

6.4

Vitamin and mineral premix10

0.3

0.3

0.3

0.3

0.3

Choline HCl

0.1

0.1

0.1

0.1

0.1

Sodium phosphate (NaH2PO4)

1.6

1.6

1.6

L-Lysine11

0.4

0.4

DL-Methionine12

0.4

0.4

0.3

Celite

1.5

1.5

1.5

1.5

1.5

Proximate composition

 Crude protein (N × 6.25)

45.2

45.3

45.1

45.5

45.4

 Crude lipid

20.1

20.1

20.2

20.3

20.2

 Starch13

6.2

6.5

7.6

8.4

8.6

 Carbohydrate14

23.2

21.2

19.5

17.8

17.8

 Moisture

4.4

5.7

5.7

4.3

4.7

 Ash

7.1

7.7

9.5

12.1

11.9

 Gross energy, MJ/kg DM

23.0

23.1

22.9

22.8

22.8

  1. 1Fishmeal Super Prime - Pesquera Diamante Peru (66.3% crude protein (CP), 11.5% crude fat (CF)). 2Fishmeal by-product Conresa 60, Conserveros Reunidos S.A. Spain (61.2% CP, 8.4% CF). 3Vegetable-protein source mixture 1 (% composition): soy protein concentrate-Soycomil, 49; wheat gluten, 29; corn gluten, 22. 4Vegetable-protein source mixture 2 (% composition): dehulled solvent extracted soybean meal, 65; defatted rapeseed meal, 35. 5ProteinX™, Protix, Dongen, The Netherlands (CP, 55.4%; CF, 20.8% as fed). 6Poultry by-product meal from Azienda Agricola Tre Valli; Verona, Italy (CP, 65.6%; CF, 14.8% as fed). 7Feeding stimulants (% composition): fish protein concentrate CPSP90- Sopropeche, France (82.6% CP), 64; Squid meal (80.3% CP), 36. 8Fish oil: from pleagic forage fish, Sopropêche, France. 9Vegetable oil mix (% composition): rapeseed oil, 56; linseed oil, 26; palm oil, 18. 10Vitamin and mineral supplement (per kg of premix): Vit. A, 2,000,000 IU; Vit D3, 200,000 IU; Vit. E 30,000 mg; Vit. K3, 2500 mg; Vit.B1, 3000 mg; Vit. B2, 3000 mg: Vit B3, 20,000 mg; Vit. B5, 10,000 mg; Vit B6, 2000 mg, Vit. B9, 1500 mg; Vit. B12, 10 mg, Biotin, 300 mg; Stay C®, 90,000 mg; Inositol, 200,000 mg; Cu, 900 mg; Fe, 6000 mg; I, 400 mg; Se, 40 mg; Zn, 7500 mg. 11L-lysine, 99%; Ajinomoto EUROLYSINE S.A.S; France. 12DL-Methionine: 99%; EVONIK Nutrition & Care GmbH; Germany. 13Calculated from the starch content of single ingredients. 14 Calculated by difference. *The ingredients were obtained from local providers by Sparos Lda