Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows

Park, Tansol; Ma, Lu; Gao, Shengtao; Bu, Dengpan; Yu, Zhongtang

doi:10.1186/s40104-022-00717-z

Journal of Animal Science and Biotechnology

Table 3 Predicted prokaryotic and fungal enzymes^* significantly (P < 0.05) reduced (as detected using LEfSe) in the ruminal microbiota of heat-stressed (HS) cows relative to pair-fed thermal neutral (PFTN) control cows

From: Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows

Enzymes	EC number	Relative abundance, %		SEM	LDA score	P-value
Enzymes	EC number	PFTN	HS	SEM	LDA score	P-value
Predicted enzymes from 16S rRNA genes
Glutamate-5-semialdehyde dehydrogenase	EC:1.2.1.41	0.100	0.077	0.005	2.056	0.021
Hydroxylamine reductase	EC:1.7.99.1	0.108	0.085	0.005	2.013	0.012
3-deoxy-7-phosphoheptulonate synthase	EC:2.5.1.54	0.105	0.065	0.011	2.273	0.036
Non-specific serine/threonine protein kinase	EC:2.7.11.1	0.280	0.244	0.011	2.199	0.046
Glutamate 5-kinase	EC:2.7.2.11	0.100	0.077	0.005	2.055	0.021
Serine-type D-Ala-D-Ala carboxypeptidase	EC:3.4.16.4	0.276	0.240	0.013	2.213	0.036
Oxaloacetate decarboxylase	EC:4.1.1.3	0.175	0.134	0.009	2.327	0.021
Asparaginyl-tRNA synthase (glutamine-hydrolyzing)	EC:6.3.5.6	0.156	0.103	0.019	2.432	0.036
Glutaminyl-tRNA synthase (glutamine-hydrolyzing)	EC:6.3.5.7	0.190	0.132	0.018	2.438	0.036
Predicted enzymes from fungal ITS1
Superoxide dismutase	EC:1.15.1.1	0.356	0.321	0.007	2.190	0.012
Cytochrome-b5 reductase	EC:1.6.2.2	0.274	0.237	0.009	2.233	0.027
Cytochrome-c oxidase	EC:1.9.3.1	0.483	0.446	0.009	2.181	0.036
Histone-lysine N-methyltransferase	EC:2.1.1.43	0.293	0.261	0.007	2.160	0.016
Phosphatidylinositol N-acetylglucosaminyltransferase	EC:2.4.1.198	0.261	0.224	0.009	2.227	0.027
Hexokinase	EC:2.7.1.1	0.156	0.132	0.005	2.049	0.006
6-phosphofructo-2-kinase	EC:2.7.1.105	0.118	0.095	0.006	2.007	0.046
Ribose-phosphate diphosphokinase	EC:2.7.6.1	0.249	0.219	0.007	2.106	0.021
Mannose-1-phosphate guanylyltransferase	EC:2.7.7.13	0.217	0.188	0.008	2.128	0.036
Ethanolaminephosphotransferase	EC:2.7.8.1	0.132	0.109	0.006	2.046	0.021
Triacylglycerol lipase	EC:3.1.1.3	0.493	0.444	0.012	2.342	0.036
Transferred entry: 4.6.1.16	EC:3.1.27.9	0.206	0.181	0.006	2.038	0.016
Alkaline phosphatase	EC:3.1.3.1	0.138	0.114	0.005	2.027	0.012
Glutamate decarboxylase	EC:4.1.1.15	0.147	0.125	0.007	2.022	0.046

Only the major predicted enzymes with a relative abundance > 0.1% for at least one of the environmental conditions were presented * is superscript

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ISSN: 2049-1891

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