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Table 3 Predicted prokaryotic and fungal enzymes* significantly (P < 0.05) reduced (as detected using LEfSe) in the ruminal microbiota of heat-stressed (HS) cows relative to pair-fed thermal neutral (PFTN) control cows

From: Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows

Enzymes EC number Relative abundance, % SEM LDA score P-value
PFTN HS
Predicted enzymes from 16S rRNA genes
 Glutamate-5-semialdehyde dehydrogenase EC:1.2.1.41 0.100 0.077 0.005 2.056 0.021
 Hydroxylamine reductase EC:1.7.99.1 0.108 0.085 0.005 2.013 0.012
 3-deoxy-7-phosphoheptulonate synthase EC:2.5.1.54 0.105 0.065 0.011 2.273 0.036
 Non-specific serine/threonine protein kinase EC:2.7.11.1 0.280 0.244 0.011 2.199 0.046
 Glutamate 5-kinase EC:2.7.2.11 0.100 0.077 0.005 2.055 0.021
 Serine-type D-Ala-D-Ala carboxypeptidase EC:3.4.16.4 0.276 0.240 0.013 2.213 0.036
 Oxaloacetate decarboxylase EC:4.1.1.3 0.175 0.134 0.009 2.327 0.021
 Asparaginyl-tRNA synthase (glutamine-hydrolyzing) EC:6.3.5.6 0.156 0.103 0.019 2.432 0.036
 Glutaminyl-tRNA synthase (glutamine-hydrolyzing) EC:6.3.5.7 0.190 0.132 0.018 2.438 0.036
Predicted enzymes from fungal ITS1
 Superoxide dismutase EC:1.15.1.1 0.356 0.321 0.007 2.190 0.012
 Cytochrome-b5 reductase EC:1.6.2.2 0.274 0.237 0.009 2.233 0.027
 Cytochrome-c oxidase EC:1.9.3.1 0.483 0.446 0.009 2.181 0.036
 Histone-lysine N-methyltransferase EC:2.1.1.43 0.293 0.261 0.007 2.160 0.016
 Phosphatidylinositol N-acetylglucosaminyltransferase EC:2.4.1.198 0.261 0.224 0.009 2.227 0.027
 Hexokinase EC:2.7.1.1 0.156 0.132 0.005 2.049 0.006
 6-phosphofructo-2-kinase EC:2.7.1.105 0.118 0.095 0.006 2.007 0.046
 Ribose-phosphate diphosphokinase EC:2.7.6.1 0.249 0.219 0.007 2.106 0.021
 Mannose-1-phosphate guanylyltransferase EC:2.7.7.13 0.217 0.188 0.008 2.128 0.036
 Ethanolaminephosphotransferase EC:2.7.8.1 0.132 0.109 0.006 2.046 0.021
 Triacylglycerol lipase EC:3.1.1.3 0.493 0.444 0.012 2.342 0.036
 Transferred entry: 4.6.1.16 EC:3.1.27.9 0.206 0.181 0.006 2.038 0.016
 Alkaline phosphatase EC:3.1.3.1 0.138 0.114 0.005 2.027 0.012
 Glutamate decarboxylase EC:4.1.1.15 0.147 0.125 0.007 2.022 0.046
  1. Only the major predicted enzymes with a relative abundance > 0.1% for at least one of the environmental conditions were presented * is superscript