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Table 2 Predicted prokaryotic and fungal enzymes* significantly (P < 0.05) enriched (as detected using LEfSe) in the ruminal microbiota of heat-stressed (HS) cows relative to pair-fed thermal neutral (PFTN) control cows

From: Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows

Enzymes EC number Relative abundance, % SEM LDA score P-value
PFTN HS
Predicted enzymes from prokaryotic 16S rRNA genes
 Malate dehydrogenase EC:1.1.1.37 0.119 0.140 0.007 2.036 0.046
 Succinate dehydrogenase (quinone) EC:1.3.5.1 0.248 0.276 0.010 2.142 0.046
 Glycine dehydrogenase (aminomethyl-transferring) EC:1.4.4.2 0.172 0.210 0.010 2.248 0.046
 Thiamine-phosphate diphosphorylase EC:2.5.1.3 0.186 0.205 0.006 2.026 0.046
 Fructokinase EC:2.7.1.4 0.137 0.159 0.005 2.005 0.036
 Galactokinase EC:2.7.1.6 0.149 0.172 0.006 2.068 0.016
 Aspartate kinase EC:2.7.2.4 0.282 0.311 0.007 2.126 0.036
 Type I site-specific deoxyribonuclease EC:3.1.21.3 0.416 0.455 0.011 2.314 0.046
 Ribonuclease Z EC:3.1.26.11 0.130 0.150 0.006 2.013 0.046
 Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase EC:3.1.4.55 0.106 0.133 0.008 2.105 0.036
 Tripeptide aminopeptidase EC:3.4.11.4 0.128 0.151 0.006 2.055 0.021
 Peptidyl-dipeptidase Dcp EC:3.4.15.5 0.185 0.222 0.011 2.248 0.046
 C-terminal processing peptidase EC:3.4.21.102 0.376 0.426 0.016 2.401 0.036
 XTP/dITP diphosphatase EC:3.6.1.66 0.271 0.299 0.010 2.173 0.046
 N-acetylmuramic acid 6-phosphate etherase EC:4.2.1.126 0.080 0.110 0.007 2.154 0.036
 Peptidylprolyl isomerase EC:5.2.1.8 1.012 1.140 0.039 2.770 0.046
 Mannose-6-phosphate isomerase EC:5.3.1.8 0.155 0.181 0.008 2.125 0.027
 Carbamoyl-phosphate synthase (glutamine-hydrolyzing) EC:6.3.5.5 0.364 0.398 0.008 2.239 0.016
Predicted fungal enzymes from fungal ITS1
 Pyridoxine 4-dehydrogenase EC:1.1.1.65 0.196 0.327 0.035 2.784 0.021
 Aryl-alcohol dehydrogenase (NADP(+)) EC:1.1.1.91 0.104 0.129 0.006 2.022 0.036
 Unspecific monooxygenase EC:1.14.14.1 0.621 1.225 0.153 3.429 0.021
 D-aspartate oxidase EC:1.4.3.1 0.080 0.104 0.006 2.030 0.027
 Glucuronosyltransferase EC:2.4.1.17 0.048 0.160 0.032 2.724 0.046
 Phospholipase D EC:3.1.4.4 0.181 0.267 0.024 2.610 0.036
  1. * Only the major predicted enzymes with a relative abundance > 0.1% for at least one of the environmental conditions were presented