Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows

Park, Tansol; Ma, Lu; Gao, Shengtao; Bu, Dengpan; Yu, Zhongtang

doi:10.1186/s40104-022-00717-z

Journal of Animal Science and Biotechnology

Table 2 Predicted prokaryotic and fungal enzymes^* significantly (P < 0.05) enriched (as detected using LEfSe) in the ruminal microbiota of heat-stressed (HS) cows relative to pair-fed thermal neutral (PFTN) control cows

From: Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows

Enzymes	EC number	Relative abundance, %		SEM	LDA score	P-value
Enzymes	EC number	PFTN	HS	SEM	LDA score	P-value
Predicted enzymes from prokaryotic 16S rRNA genes
Malate dehydrogenase	EC:1.1.1.37	0.119	0.140	0.007	2.036	0.046
Succinate dehydrogenase (quinone)	EC:1.3.5.1	0.248	0.276	0.010	2.142	0.046
Glycine dehydrogenase (aminomethyl-transferring)	EC:1.4.4.2	0.172	0.210	0.010	2.248	0.046
Thiamine-phosphate diphosphorylase	EC:2.5.1.3	0.186	0.205	0.006	2.026	0.046
Fructokinase	EC:2.7.1.4	0.137	0.159	0.005	2.005	0.036
Galactokinase	EC:2.7.1.6	0.149	0.172	0.006	2.068	0.016
Aspartate kinase	EC:2.7.2.4	0.282	0.311	0.007	2.126	0.036
Type I site-specific deoxyribonuclease	EC:3.1.21.3	0.416	0.455	0.011	2.314	0.046
Ribonuclease Z	EC:3.1.26.11	0.130	0.150	0.006	2.013	0.046
Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase	EC:3.1.4.55	0.106	0.133	0.008	2.105	0.036
Tripeptide aminopeptidase	EC:3.4.11.4	0.128	0.151	0.006	2.055	0.021
Peptidyl-dipeptidase Dcp	EC:3.4.15.5	0.185	0.222	0.011	2.248	0.046
C-terminal processing peptidase	EC:3.4.21.102	0.376	0.426	0.016	2.401	0.036
XTP/dITP diphosphatase	EC:3.6.1.66	0.271	0.299	0.010	2.173	0.046
N-acetylmuramic acid 6-phosphate etherase	EC:4.2.1.126	0.080	0.110	0.007	2.154	0.036
Peptidylprolyl isomerase	EC:5.2.1.8	1.012	1.140	0.039	2.770	0.046
Mannose-6-phosphate isomerase	EC:5.3.1.8	0.155	0.181	0.008	2.125	0.027
Carbamoyl-phosphate synthase (glutamine-hydrolyzing)	EC:6.3.5.5	0.364	0.398	0.008	2.239	0.016
Predicted fungal enzymes from fungal ITS1
Pyridoxine 4-dehydrogenase	EC:1.1.1.65	0.196	0.327	0.035	2.784	0.021
Aryl-alcohol dehydrogenase (NADP⁽⁺⁾)	EC:1.1.1.91	0.104	0.129	0.006	2.022	0.036
Unspecific monooxygenase	EC:1.14.14.1	0.621	1.225	0.153	3.429	0.021
D-aspartate oxidase	EC:1.4.3.1	0.080	0.104	0.006	2.030	0.027
Glucuronosyltransferase	EC:2.4.1.17	0.048	0.160	0.032	2.724	0.046
Phospholipase D	EC:3.1.4.4	0.181	0.267	0.024	2.610	0.036

^* Only the major predicted enzymes with a relative abundance > 0.1% for at least one of the environmental conditions were presented

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ISSN: 2049-1891

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