Fig. 7

Effects of NMN supplementation on the mitochondrial distribution and function in heat-stressed porcine oocytes. A Representative images of mitochondrial distribution in control, heat-stressed and NMN-supplemented oocytes at metaphase II stage. Scale bar, 30 μm. B The fluorescence intensity of mitochondrial signals was quantified in control, heat-stressed and NMN-supplemented oocytes. C Mitochondrial membrane potential (ΔΨm) was detected by JC-1 staining in control, heat-stressed and NMN-supplemented oocytes at metaphase II stage. Red, high ΔΨm; Green, low ΔΨm. Scale bar, 30 μm. D The ratio of red to green fluorescence intensity was calculated in control, heat-stressed and NMN-supplemented oocytes. Data in (B) and (D) were presented as mean value (mean ± SD) of at least three independent experiments. ***P < 0.001