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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

Fig. 5

Effects of NMN supplementation on the distribution of CGs and ovastacin in heat-stressed porcine oocytes. A Representative images of CG localization in control, heat-stressed and NMN-supplemented oocytes at metaphase II stage. Zona pellucida was removed from oocytes before LCA-FITC staining because of the non-specific binding. Scale bar, 30 μm. B The fluorescence intensity of CG signals was quantified in control, heat-stressed and NMN-supplemented oocytes. C Representative images of ovastacin localization in control, heat-stressed and NMN-supplemented oocytes at metaphase II stage. Scale bar, 30 μm. D The fluorescence intensity of ovastacin signals was quantified in control, heat-stressed and NMN-supplemented oocytes. Data in (B) and (D) were presented as mean value (mean ± SD) of at least three independent experiments. **P < 0.01, ***P < 0.001

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