Fig. 3

Effects of NMN supplementation on the microtubule stability in heat-stressed porcine oocytes. A Representative images of acetylated α-tubulin in control, heat-stressed and NMN-supplemented oocytes at metaphase I stage. Scale bar, 5 μm. B The fluorescence intensity of acetylated α-tubulin was quantified in control, heat-stressed and NMN-supplemented oocytes. Data were presented as mean value (mean ± SD) of at least three independent experiments. ***P < 0.001. C Representative images of microtubule fibers after nocodazole treatment in control, heat-stressed and NMN-supplemented oocytes. Oocytes were placed in cold culture media at 4 °C for 5 min, followed by the immunostaining for α-tubulin. Scale bar, 5 μm