Fig. 2

Effects of NMN supplementation on the spindle assembly and chromosome alignment in heat-stressed porcine oocytes. A Representative images of spindle/chromosome structure in control, heat-stressed and NMN-supplemented oocytes at metaphase I stage. Scale bar, 5 μm. B The rate of disorganized spindles was recorded in control, heat-stressed and NMN-supplemented oocytes. C The rate of misaligned chromosomes was recorded in control, heat-stressed and NMN-supplemented oocytes. Data in (B) and (C) were presented as mean percentage (mean ± SEM) of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001