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Fig. 9 | Journal of Animal Science and Biotechnology

Fig. 9

From: Tauroursodeoxycholic acid (TUDCA) improves intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets

Fig. 9

TGR5-MLCK signaling pathway was involved in TUDCA-improved epithelial barrier impairment in E. coli-treated IPEC-J2 cells. A-C Western blotting measurements of the protein levels of MLCK, p-MLC and MLC in IPEC-J2 cells after treatment with TUDCA and/or E. coli and/or TGR5 siRNA. Tubulin was used as the loading control (n = 4) P < 0.05 and P < 0.01. NS = not significant. D and E Western blotting measurements of the protein levels of MLCK, p-MLC/MLC, ZO-1, OCC and Claudin-1 in the presence of E. coli and/or ML-7. Tubulin was used as the loading control (n = 4). P < 0.05 versus the control group. #P < 0.05 versus the E. coli group

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