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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens

Fig. 5

Investigation of histone modification and chromatin status of DAZL::GFP cells at E12, E16, and hatch. A Histone acetylation at E12, E16, and hatch. DAZL::GFP cells are stained with anti-H3K9ac and anti-H3K14ac antibodies and with DAPI. Scale bars, 20 μm. B Expression of heterochromatin-marker histone-H3K9 trimethylation at E12, E16, and hatch. DAZL::GFP cells are stained with anti-trimethyl-H3K9 antibody and DAPI (for nuclei). Scale bars, 20 μm. C Confirmation of chromatin structure of left testes germ cells at E12, E16, and hatch by transmission electron microscopy. Scale bars, 5 μm (upper row) and 1 μm (bottom row)

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891