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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens

Fig. 3

Examination of transcriptional changes in male germ cells between time points (E12, E16, and hatch). A Schematic representation of germ cell mitotic arrest in embryonic testes. B Volcano plots displaying the DEGs between groups (E12 vs. hatch, E12 vs. E16, and E16 vs. hatch). Red dots indicate log2 FC > 1, and blue dots indicate log2 FC < –1. Gray dots are DEGs with log2 FC between − 1 and 1, and horizontal dotted lines represent FDRs of 0.05. The numbers of red dots and blue dots are shown at the bottom of the graph. C Heatmaps showing top DEGs in E12 vs. hatch, E12 vs. E16, and E16 vs. hatch. Among the DEGs corresponding to |log2 FC| > 1 and FDR < 0.05, the 50 most upregulated and 50 most downregulated DEGs are extracted on the basis of FDR values. Fewer than 50 DEGs are shown in the heatmap of E16 vs. hatch because the heatmap includes only annotated genes. Red boxes and arrow heads indicate epigenetic-modification-related genes. D K-means clustering of DEGs for E12 vs. hatch, E12 vs. E16, and E16 vs. hatch, and Gene Ontology enrichment (biological process) analysis for each cluster based on K-means clustering using the PANTHER database

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