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Table 1 Primers used in qRT-PCR

From: The m6A methylation regulates gonadal sex differentiation in chicken embryo

Gene Primer Accession number Annealing temperature, °C Fragment size, bp
β-actin F:5′- GAGAAATTGTGCGTGACATCA- 3' NM_205518 54/56 152
R:5′- CCTGAACCTCTCATTGCCA- 3'
YTHDC2 F: 5′- GATGTCGTTTCCTTCGTC- 3' XM_004949271 54 187
R: 5′- CTGTTTCGTTCTGGGTGT- 3'
FTO F: 5′- GGGACATAGAGACACCTG- 3' NM_001185147 54 253
R: 5′- GCAGTTTCCAGTGATTTC- 3'
METTL3 F: 5′- TAAGTTCGCCGTGGTGAT- 3' XM_025145967 54 181
R: 5′- CCAAAGGTTCAGGCATTC- 3'
ALKBH5 F: 5′- CGCTGCGGAACAAGTATT- 3' NM_001257201 54 282
R: 5′- TGAAGAACGACACGGAGA- 3'
DMRT1 F: 5′- GCAACCACGGCTACTCCTCGC- 3' NM_001101831 56 146
R: 5′- TTCCTGGGCTTGCTGCCTCCT- 3'
HEMGN F: 5′- AACCACAGCCAAACCCTC- 3' XM_430508 56 185
R: 5′- CAGCATATCCTCTTCACCC- 3'
AMH F: 5′- GGATGGAGGTGCCCCTCTGT- 3' NM_205030 56 129
R: 5′- GCAGCATCACCCTCAGGTGG- 3'
SOX9 F: 5′- AGTACCCGCATCTGCACAA- 3' NM_204281 56 161
R: 5′- CCTCCTGCGTGGTTGGTA- 3'
FOXL2 F: 5′- CTGATCGCCATGGCCATACG- 3' NM_001012612 56 127
R: 5′- GGCGGATGCTGTTCTGCCA- 3'
CYP19A1 F: 5′- GGAATTGGGCCTCTCATTTC- 3' NM_001364699 56 154
R: 5′- CGTGAAATACGCTGGAGGAT- 3'
  1. Abbreviations: F Forward, R Reverse