From: The m6A methylation regulates gonadal sex differentiation in chicken embryo
Gene | Primer | Accession number | Annealing temperature, °C | Fragment size, bp |
---|---|---|---|---|
β-actin | F:5′- GAGAAATTGTGCGTGACATCA- 3' | NM_205518 | 54/56 | 152 |
R:5′- CCTGAACCTCTCATTGCCA- 3' | ||||
YTHDC2 | F: 5′- GATGTCGTTTCCTTCGTC- 3' | XM_004949271 | 54 | 187 |
R: 5′- CTGTTTCGTTCTGGGTGT- 3' | ||||
FTO | F: 5′- GGGACATAGAGACACCTG- 3' | NM_001185147 | 54 | 253 |
R: 5′- GCAGTTTCCAGTGATTTC- 3' | ||||
METTL3 | F: 5′- TAAGTTCGCCGTGGTGAT- 3' | XM_025145967 | 54 | 181 |
R: 5′- CCAAAGGTTCAGGCATTC- 3' | ||||
ALKBH5 | F: 5′- CGCTGCGGAACAAGTATT- 3' | NM_001257201 | 54 | 282 |
R: 5′- TGAAGAACGACACGGAGA- 3' | ||||
DMRT1 | F: 5′- GCAACCACGGCTACTCCTCGC- 3' | NM_001101831 | 56 | 146 |
R: 5′- TTCCTGGGCTTGCTGCCTCCT- 3' | ||||
HEMGN | F: 5′- AACCACAGCCAAACCCTC- 3' | XM_430508 | 56 | 185 |
R: 5′- CAGCATATCCTCTTCACCC- 3' | ||||
AMH | F: 5′- GGATGGAGGTGCCCCTCTGT- 3' | NM_205030 | 56 | 129 |
R: 5′- GCAGCATCACCCTCAGGTGG- 3' | ||||
SOX9 | F: 5′- AGTACCCGCATCTGCACAA- 3' | NM_204281 | 56 | 161 |
R: 5′- CCTCCTGCGTGGTTGGTA- 3' | ||||
FOXL2 | F: 5′- CTGATCGCCATGGCCATACG- 3' | NM_001012612 | 56 | 127 |
R: 5′- GGCGGATGCTGTTCTGCCA- 3' | ||||
CYP19A1 | F: 5′- GGAATTGGGCCTCTCATTTC- 3' | NM_001364699 | 56 | 154 |
R: 5′- CGTGAAATACGCTGGAGGAT- 3' |