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Table 1 Primers used in qRT-PCR

From: The m6A methylation regulates gonadal sex differentiation in chicken embryo

Gene

Primer

Accession number

Annealing temperature, °C

Fragment size, bp

β-actin

F:5′- GAGAAATTGTGCGTGACATCA- 3'

NM_205518

54/56

152

R:5′- CCTGAACCTCTCATTGCCA- 3'

YTHDC2

F: 5′- GATGTCGTTTCCTTCGTC- 3'

XM_004949271

54

187

R: 5′- CTGTTTCGTTCTGGGTGT- 3'

FTO

F: 5′- GGGACATAGAGACACCTG- 3'

NM_001185147

54

253

R: 5′- GCAGTTTCCAGTGATTTC- 3'

METTL3

F: 5′- TAAGTTCGCCGTGGTGAT- 3'

XM_025145967

54

181

R: 5′- CCAAAGGTTCAGGCATTC- 3'

ALKBH5

F: 5′- CGCTGCGGAACAAGTATT- 3'

NM_001257201

54

282

R: 5′- TGAAGAACGACACGGAGA- 3'

DMRT1

F: 5′- GCAACCACGGCTACTCCTCGC- 3'

NM_001101831

56

146

R: 5′- TTCCTGGGCTTGCTGCCTCCT- 3'

HEMGN

F: 5′- AACCACAGCCAAACCCTC- 3'

XM_430508

56

185

R: 5′- CAGCATATCCTCTTCACCC- 3'

AMH

F: 5′- GGATGGAGGTGCCCCTCTGT- 3'

NM_205030

56

129

R: 5′- GCAGCATCACCCTCAGGTGG- 3'

SOX9

F: 5′- AGTACCCGCATCTGCACAA- 3'

NM_204281

56

161

R: 5′- CCTCCTGCGTGGTTGGTA- 3'

FOXL2

F: 5′- CTGATCGCCATGGCCATACG- 3'

NM_001012612

56

127

R: 5′- GGCGGATGCTGTTCTGCCA- 3'

CYP19A1

F: 5′- GGAATTGGGCCTCTCATTTC- 3'

NM_001364699

56

154

R: 5′- CGTGAAATACGCTGGAGGAT- 3'

  1. Abbreviations: F Forward, R Reverse