Fig. 4From: Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducksVerification of hepatic proteomic analyses in Pekin ducks by Western blot and real-time PCR. The mRNA abundances were determined by real-time PCR analysis (a) and protein expression determined by Western Blot (b) in liver; (c, d) fold change of selected proteins between real-time PCR, Western blot validation and proteomics analysis. Results are presented as means with plus error bars (standard deviation). Differences were assessed by Student’s t test (n = 6) and denoted as follows: * P < 0.05; ** P < 0.01; *** P < 0.001. Met-D, Met-deficient; Met-A, Met-adequate. FABP1 (L-FABP), Fatty acid-binding protein, liver; ACBP, Acyl-CoA-binding protein; ACSL5, Acyl coenzyme A long-chain 5 synthetase; FATP5, Fatty acid transport protein 5; LDHA, Lactate dehydrogenase A chain; FASN, Fatty acid synthase; NDUFS1, NADH:ubiquinone oxidoreductase core subunit S1; ACADM, Acyl-CoA dehydrogenase medium chain; ALB, AlbuminBack to article page