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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks

Fig. 4

Verification of hepatic proteomic analyses in Pekin ducks by Western blot and real-time PCR. The mRNA abundances were determined by real-time PCR analysis (a) and protein expression determined by Western Blot (b) in liver; (c, d) fold change of selected proteins between real-time PCR, Western blot validation and proteomics analysis. Results are presented as means with plus error bars (standard deviation). Differences were assessed by Student’s t test (n = 6) and denoted as follows: * P < 0.05; ** P < 0.01; *** P < 0.001. Met-D, Met-deficient; Met-A, Met-adequate. FABP1 (L-FABP), Fatty acid-binding protein, liver; ACBP, Acyl-CoA-binding protein; ACSL5, Acyl coenzyme A long-chain 5 synthetase; FATP5, Fatty acid transport protein 5; LDHA, Lactate dehydrogenase A chain; FASN, Fatty acid synthase; NDUFS1, NADH:ubiquinone oxidoreductase core subunit S1; ACADM, Acyl-CoA dehydrogenase medium chain; ALB, Albumin

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