Fig. 3

Partial Least Squares Discriminant Analysis (PLS-DA) 2D score plot of the metabolites in colon digesta showed separated clusters between the TRA and REC groups on d 5 (A) and 11 (B) post-inoculation. ● = CON (Control); ● = TRA (Trace amounts of antibiotic); ● = REC (Label-recommended dose of antibiotic). Shaded areas in different colors represent in 95% confidence interval