Skip to main content

Table 3 Effect of slow-release urea supplementation on fermentation pH and characteristics in Rusitec

From: Effect of commercial slow-release urea product on in vitro rumen fermentation and ruminal microbial community using RUSITEC technique

Item Treatmenta   Pb
Control U28 U56 MU35 SEM L Q C vs. MU35
pH 6.81 6.80 6.80 6.77 0.01 0.25 0.47 0.03
NH3-N, mmol/L 7.70 9.31 10.25 8.92 0.37 0.01 0.39 0.03
Total VFA, mmol/L 78.5 80.2 79.3 85.7 2.86 0.21 0.65 0.08
% of total VFA
 Acetate (A) 46.1 45.6 46.4 47.2 0.57 0.57 0.16 0.16
 Propionate (P) 26.9 25.7 26.0 26.5 0.21 0.01 0.01 0.16
 Butyrate 14.9 15.5 15.2 14.2 0.24 0.38 0.13 0.04
 BCVFA3 2.8 3.2 2.9 3.2 0.09 0.87 0.01 0.02
 Valerate 7.8 8.4 7.9 7.4 0.25 0.63 0.03 0.22
 Caproate 1.3 1.6 1.5 1.4 0.05 0.02 0.01 0.08
 A:P 1.69 1.77 1.78 1.77 0.03 0.01 0.24 0.02
Total VFA, mmol/d 50.5 53.1 52.0 55.9 2.05 0.13 0.18 0.06
Protozoa, × 104/mL 2.4 2.9 2.8 2.8 0.24 0.91 0.17 0.24
  1. aControl (no SRU), U28 = control plus 0.28% SRU, U56 = control plus 0.56% SRU, and MU35 = control diet was modified by partially replacing soybean protein with 0.35% SRU (DM basis);
  2. bL, Q = linear and quadratic effects of SRU (control, U28 and U56); C vs. MU35 = Contrast between Control and MU35
  3. cBCVFA = Branched-chain volatile fatty acids (isobutyrate + isovalerate)