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Fig. 9 | Journal of Animal Science and Biotechnology

Fig. 9

From: miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

Fig. 9

Effect of miR-30a-5p on oxidative stress of granulosa cells. (A, B) Flow cytometry analysis of mitochondrial cell membrane potential after the interference or overexpression of miR-30a-5p. The statistics of the depolarization rate of the mitochondrial membrane potential (n = 3). (C, D) ROS levels were analyzed using flow cytometry after the interference or overexpression of miR-30a-5p. The statistics of the number of ROS positive cells (n = 3). (E, F) The levels of MDA and SOD in the cell supernatant after interference or overexpression of miR-30a-5p (n = 6). Data are presented as mean ± SEM. * P < 0.05, **P < 0.01 vs. NC

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891